Obiettivo Epithelia have the essential role of acting as a barrier that protects living organisms and its organs from the surrounding milieu. Therefore, it is crucial for epithelial tissues to have robust ways of maintaining its integrity despite the frequent damage caused by normal cell turnover, inflammation and injury. All epithelia have some capacity to repair themselves, however, the wound-healing process differs dramatically between the developmental stage and type of tissue involved. In this project we will focus on investigating the capacity that several simple epithelial tissues have to reseal small discontinuities very rapidly and efficiently. This repair mechanism that we call epithelial resealing is based on the contraction of an actomyosin purse string in the leading edge cells around the wound margin. Epithelial resealing seems to be a fundamental repair mechanism, acting in several types of simple embryonic and adult epithelia, in both vertebrates and invertebrates. The cell biology of epithelial resealing has started to be understood but there are still many open questions and the signalling cascades that regulate this process are largely unknown. We propose to investigate epithelial resealing using a combination of genetics and high resolution live imaging. The Drosophila embryonic epithelium will be our primary model system and we will start by characterizing in detail novel genes involved in resealing that have been identified in a pilot screen previously performed in the laboratory. We will also perform a new RNAi genetic screen based on a very large collections of transgenic lines to completely unravel the signalling network that controls epithelial resealing. In order to investigate how conserved in vertebrates are the epithelial resealing mechanisms, we will establish epithelial wounding assays in zebrafish simple epithelial tissues and we will study, in this vertebrate model system, the molecular mechanisms that we will uncover using Drosophila. Campo scientifico natural sciencesbiological sciencesgeneticsnatural sciencesbiological sciencescell biologynatural sciencesbiological scienceszoologyinvertebrate zoology Parole chiave Drosophila Wound healing morphogenesis Programma(i) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Argomento(i) ERC-SG-LS1 - ERC Starting Grant - Molecular and Structural Biology and Biochemistry Invito a presentare proposte ERC-2007-StG Vedi altri progetti per questo bando Meccanismo di finanziamento ERC-SG - ERC Starting Grant Istituzione ospitante FUNDACAO CALOUSTE GULBENKIAN Contributo UE € 702 628,00 Indirizzo AVENIDA BERNA 45A 1067-001 LISBOA Portogallo Mostra sulla mappa Regione Continente Área Metropolitana de Lisboa Área Metropolitana de Lisboa Tipo di attività Research Organisations Ricercatore principale Antonio Alfredo Coelho Jacinto (Prof.) Contatto amministrativo José Mário Leite (Mr.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato Beneficiari (2) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto FUNDACAO CALOUSTE GULBENKIAN Portogallo Contributo UE € 702 628,00 Indirizzo AVENIDA BERNA 45A 1067-001 LISBOA Mostra sulla mappa Regione Continente Área Metropolitana de Lisboa Área Metropolitana de Lisboa Tipo di attività Research Organisations Ricercatore principale Antonio Alfredo Coelho Jacinto (Prof.) Contatto amministrativo José Mário Leite (Mr.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato INSTITUTO DE MEDICINA MOLECULAR JOAO LOBO ANTUNES Partecipazione conclusa Portogallo Contributo UE € 447 372,00 Indirizzo AVENIDA PROF EGAS MONIZ 1649 028 Lisboa Mostra sulla mappa Regione Continente Área Metropolitana de Lisboa Área Metropolitana de Lisboa Tipo di attività Research Organisations Contatto amministrativo Margarida Pinto Gago (Dr.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato
