Objetivo Difficulties encountered in tissue engineering invited the use of perfusion bioreactors to deliver essential nutrients to cells within tissue-engineered constructs. Despite the increased nutrient transport achieved, solute perfusion caused harmful mechanical stresses to the cells. Interestingly, some bioreactors are employed to mechanically stimulate cells, improving their biological functions. However, stresses associated with perfusion bioreactors are counterproductive. Currently, to address this issue, perfusion rate is lowered to minimise mechanical implications to the cells. Consequently, nutrient delivery is sacrificed. Therefore, this project aims to balance the nutritional advantage offered by perfusion, and its associate cell-death. By doing so, essential nutrients may be perfused to all regions of tissue-engineered constructs, at flow rates whose mechanical implications are harmless, or even encouraging to cells. To alleviate the costly and iterative experiments necessary to achieve these aims, the use of computational modelling will be central to the project, and incidentally, forms the training objective of the proposal. However, key findings will be corroborated with laboratory experiments. Combining techniques associated with cell biology, material science, and mechanical engineering. The specific objectives of the proposed study are to: Model the fluid flow-induced deformation of cells within tissue-engineered constructs. Using fluorescent staining, corroborate relationship between fluid flow and cellular deformation. Design a system to mechanically deform cells without fluid flow. Investigate the influence of cellular deformation, with and without fluid flow to their viability and biological activities. Extrapolate the individual contributions of fluid flow and mechanical deformation. Determine theoretically, and experimentally, useful ranges of fluid flow and mechanical deformation, conducive to developing functional neo-tissues in vitro. Ámbito científico engineering and technologyenvironmental biotechnologybioremediationbioreactorsnatural sciencesbiological sciencescell biologymedical and health sciencesmedical biotechnologytissue engineeringengineering and technologymechanical engineering Palabras clave Biomaterials Engineering Biomechanics Biomedical Engineering Bioreactors Biotechnology Health Sciences Musculoskeletal diseases Regenerative Medicine Tissue Engineering Programa(s) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Tema(s) PEOPLE-2007-2-1.IEF - Marie Curie Action: "Intra-European Fellowships for Career Development" Convocatoria de propuestas FP7-PEOPLE-2007-2-1-IEF Consulte otros proyectos de esta convocatoria Régimen de financiación MC-IEF - Intra-European Fellowships (IEF) Coordinador CHARITE - UNIVERSITAETSMEDIZIN BERLIN Aportación de la UE € 158 694,85 Dirección Chariteplatz 1 10117 Berlin Alemania Ver en el mapa Región Berlin Berlin Berlin Tipo de actividad Higher or Secondary Education Establishments Contacto administrativo Georg Duda (Prof.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos