Objective
Iron is an essential nutrient for virtually all living organisms and host iron availability plays a critical role in the host–pathogen relationship. Without effective mechanisms for acquisition, transport and utilization of iron, no organism especially parasites with high iron requirements, can survive. However, our knowledge about intracellular iron metabolism in eukaryotic pathogenic microorganisms is poor; it is not known, how iron is stored in the cell or how it is utilized. In the proposed project, we will investigate cytosolic iron metabolism in three unicellular eukaryotic pathogens: Candida albicans, Trichomonas vaginalis and Giardia intestinalis. We will use two different proteomic methods to identify proteins involved in iron metabolism: 1) 2D gel electrophoresis of cytosolic fractions from cells grown under iron-rich and iron-restricted conditions and 2) separation of 55Fe radiolabeled cytosolic iron complexes by liquid chromatography followed by native electrophoresis. Obtained proteins/protein complexes will be identified by Matrix-assisted laser desorption/ionization mass spectrometry and liquid chromatography-tandem mass spectrometry. We will test the cellular localization and the effect of the identified proteins on iron metabolism by overexpression in pathogens and yeast. The project has great potential to discover new mechanisms of iron metabolism as well as identify new therapeutic approaches for the treatment of human pathogens. This multidisciplinary project investigating different pathogenic organisms and using a broad range of state-of-the-art techniques and approaches will allow a young researcher with outstanding track record and potential to return to Europe, integrate into European scientific community, consolidate his research maturity and provide him with a strong background for establishing his own laboratory in later stages of his career.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences chemical sciences electrochemistry electrophoresis
- natural sciences chemical sciences analytical chemistry mass spectrometry
- natural sciences biological sciences microbiology
- natural sciences physical sciences optics laser physics
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2007-2-1-IEF
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
75794 Paris
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.