Cel Human embryonic stem cells (hESC) are pluripotent cells derived from blastocyst stage early embryos. Since the original publication a vast number of studies have described the differentiation of hESC and their potential in regenerative medicine. It is now well known that hESC might be used in cell replacement therapies in a number of severe degenrative diseases like Type I diabetes or Parkinson’s disease. In addition, hESCs and their derivatives could be used as tools in discovering new drugs and in understanding mechanisms of genetic diseases. HESC applications could also be used in toxicological studies reducing the need of animal experiments. Genetic manipulation of hESC will be a crucial issue in a variety of future studies. To elucidate the role of any given gene for example in directed differentiation or pathogenesis the loss- and gain-of gene function approaches are of major importance. Also, considering the potential therapeutic applications of the hESC their genetic modifications may be necessary. The first part of this proposal is designed to improve and test novel efficient and safe transposon-based gene transfer method in generating conditional transgenic hESC lines. Of special interest is the use of insulator elements around transgene to assure nonbiased expression of the integrated sequences. Several reports have indicated that in long term hESC cultures the cells aquire chromosomal aberrations. The observed karyotypic changes probably reflect the progressive adaptation of self-renewing cells to their culture conditions. This process has parallels with malignant transformation. Changes observed in hESC in culture reflect tumorigenic events that occur in vivo, particularly in testicular germ cell tumors (TGCT). A recent report indicated the formation of a chromosomal homogeneous staining region (HSR) in some hESC lines, a feature almost a hallmark of cancer cells. The HSR represents an amplified small chromosomal region most likely containing gene Dziedzina nauki medical and health sciencesmedical biotechnologygenetic engineeringgene therapymedical and health sciencesclinical medicineendocrinologydiabetesmedical and health sciencesmedical biotechnologycells technologiesstem cellsmedical and health sciencesbasic medicineneurologyparkinsonmedical and health sciencesclinical medicineembryology Słowa kluczowe adaptation human embryonic stem cells self-renewal transgenic Program(-y) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Temat(-y) PEOPLE-2007-2-1.IEF - Marie Curie Action: "Intra-European Fellowships for Career Development" Zaproszenie do składania wniosków FP7-PEOPLE-2007-2-1-IEF Zobacz inne projekty w ramach tego zaproszenia System finansowania MC-IEF - Intra-European Fellowships (IEF) Koordynator THE UNIVERSITY OF SHEFFIELD Wkład UE € 243 268,29 Adres FIRTH COURT WESTERN BANK S10 2TN Sheffield Zjednoczone Królestwo Zobacz na mapie Region Yorkshire and the Humber South Yorkshire Sheffield Rodzaj działalności Higher or Secondary Education Establishments Kontakt administracyjny Deborah Mcclean (Ms.) Linki Kontakt z organizacją Opens in new window Strona internetowa Opens in new window Koszt całkowity Brak danych