Objective
DNA single-strand breaks (SSBs) can arise directly by the action of free radicalsor indirectly as intermediates of base excision repair or topoisomerase I activity. Moreover, replication can convert a SSB into a double-strand break (DSB), one of the most severe DNA lesions that can occur in living cells. Single-strand break repair (SSBR) is therefore crucial to ensure cell survival and the maintenance of genome stability. In addition, SSBR defects have been associated with some neurodegenerative diseases.The main goal of this project is to study the molecular mechanism/s of SSBR in human cells. Our primary focus will be to develop a novel system to induce a site-specific SSB in human cells. This will allow, for the first time, direct analysis of the assembly/disassembly of protein complexes at the SSB reaction by ChIP analyses, in addition to measuring the repair event itself. For example, we will examine the sequence of recruitment of the individual SSBR proteins, as well as identifying which proteins are required for assembly/disassembly/remodelling of protein complexes at chromosomal SSBs. This approach will also provide a system to examine possible SSBR defects in neurodegenerative diseases. We are particullary interested in defining the molecular role of APLF (aprataxin and PNK like factor), a SSBR protein recently identified in this laboratory.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences genetics genomes
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2007-2-1-IEF
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
BN1 9RH Brighton
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.