Cel DNA single-strand breaks (SSBs) can arise directly by the action of free radicalsor indirectly as intermediates of base excision repair or topoisomerase I activity. Moreover, replication can convert a SSB into a double-strand break (DSB), one of the most severe DNA lesions that can occur in living cells. Single-strand break repair (SSBR) is therefore crucial to ensure cell survival and the maintenance of genome stability. In addition, SSBR defects have been associated with some neurodegenerative diseases.The main goal of this project is to study the molecular mechanism/s of SSBR in human cells. Our primary focus will be to develop a novel system to induce a site-specific SSB in human cells. This will allow, for the first time, direct analysis of the assembly/disassembly of protein complexes at the SSB reaction by ChIP analyses, in addition to measuring the repair event itself. For example, we will examine the sequence of recruitment of the individual SSBR proteins, as well as identifying which proteins are required for assembly/disassembly/remodelling of protein complexes at chromosomal SSBs. This approach will also provide a system to examine possible SSBR defects in neurodegenerative diseases. We are particullary interested in defining the molecular role of APLF (aprataxin and PNK like factor), a SSBR protein recently identified in this laboratory. Dziedzina nauki natural sciencesbiological sciencesgeneticsDNAnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesbiological sciencesgeneticsgenomes Słowa kluczowe Genome stability Health sciences Mammalian Cell Biology Molecular Biology Neurodegenerative disease Single-strand break repair Program(-y) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Temat(-y) PEOPLE-2007-2-1.IEF - Marie Curie Action: "Intra-European Fellowships for Career Development" Zaproszenie do składania wniosków FP7-PEOPLE-2007-2-1-IEF Zobacz inne projekty w ramach tego zaproszenia System finansowania MC-IEF - Intra-European Fellowships (IEF) Koordynator THE UNIVERSITY OF SUSSEX Wkład UE € 178 307,05 Adres SUSSEX HOUSE FALMER BN1 9RH Brighton Zjednoczone Królestwo Zobacz na mapie Region South East (England) Surrey, East and West Sussex Brighton and Hove Rodzaj działalności Higher or Secondary Education Establishments Kontakt administracyjny Peter Graham Brooks (Mr.) Linki Kontakt z organizacją Opens in new window Strona internetowa Opens in new window Koszt całkowity Brak danych
