Objective
Dendritic cells (DCs) are specialized antigen-presenting cells (APCs) that have a dominant role in initiating T-cell responses. The skin is populated by at least two subsets of DCs: Langerhans cells (LCs), which form a contiguous network throughout the epidermis, and dermal DC. According to the conventional paradigm, LCs perform a sentinel function by detecting skin pathogens and integrating this information to T cells in the lymph nodes (LNs). However, recent data have highlighted only the importance of dermal DC in skin immunity, while the role of LCs remains an enigma. The arrival of skin DCs to the LNs and activation of CD8+ T cells are thought to take place within 24 hrs after exposure to an antigen. We have recently described that following intradermal immunization with plasmid DNA, CD8+ T cell priming initiates 4 days post-immunization and reaches its maximal level on day 15. We also showed that arrival of APCs from the skin occurs 3 days after the immunization and proceeds for almost two weeks. Such pattern of migration is consistent with the reported migration kinetics of LCs but not of dermal DCs that enter the LN within the first 24 hrs post-immunization. We recently generated unpublished data demonstrating that T-cell priming correlates with the late arrival of CD11c+DEC205med cells (LCs) and not with that of CD11c+DEC205hi cells (dermal DCs) that are first to enter the LNs. This has led us to the hypothesis that LCs might play an important role in T-cell priming during intradermal DNA immunization. Using cellular immunology approaches and langerin-DTR and CD11c-DTR transgenic mice, we will address this issue by performing experiments falling under three specific aims. (i) Characterizing the type of skin DCs involve in antigen presentation. (ii) Identifying the factors leading to the slow-rate antigen presenting activity. (iii) Assessing the quality of the elicited immunity. These complementary aims should reveal a novel role for LCs in T-cell priming.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- medical and health sciences basic medicine immunology immunisation
- natural sciences biological sciences genetics DNA
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-IRG-2008
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
91904 JERUSALEM
Israel
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.