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Content archived on 2024-05-29

Conformational Dynamics of Proteins by Solid-State NMR

Objective

Conformational dynamics are intimately linked to the biological activity of proteins, thereby regulating the essential processes of life. In particular protein motion plays an essential role in catalysis, allowing conformational rearrangements to align key catalytic amino acids and in ligand binding allowing the entry of co-factors into areas that would otherwise be inaccessible. Mobility also plays a major role in the thermodynamic stability of functional states; in molecular recognition processes that often involve disorder-to-order transitions; and in allostery and molecular signalling, where correlated molecular motions can transmit information between distant sites in a protein. Dynamic transitions also mediate the onset of pathological malfunction and disease. NMR spectroscopy is uniquely suited to study a large number of these dynamic processes, resolving detailed and important site-specific information about motions spanning a vast range of time scales in both folded and unfolded proteins, and in both the liquid and the solid phase. We believe that the key to understanding the complex relationship between protein dynamics and molecular function and malfunction requires an accurate description of the behavior of proteins in their different phases and forms, and an understanding of the transition between different states. In this study we are interested in developing a self-consistent framework for measuring and describing protein dynamics in the solid state. To this end in this project we propose to develop a number of NMR methods extending substantially the scope of accessible protein motions in solids. This work is a part of concerted collaborative effort that couples for the first time NMR data from both solid and solutions with complementary data from elastic incoherent neutron scattering and molecular simulation in order to provide a general consistent picture of dynamical processes in proteins.

Fields of science (EuroSciVoc)

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Keywords

Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)

Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

FP7-PEOPLE-IRG-2008
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Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

MC-IRG - International Re-integration Grants (IRG)

Coordinator

ECOLE NORMALE SUPERIEURE DE LYON
EU contribution
€ 75 000,00
Address
PARVIS RENE DESCARTES 15
69342 Lyon
France

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Region
Auvergne-Rhône-Alpes Rhône-Alpes Rhône
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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