Objective Protein dynamics is essentially linked to protein function, aggregation, and folding. Time-resolved spectroscopy is specially suited to reveal these dynamics, counterbalancing the static pure-structural information provided by X-ray crystallographic structures. In equilibrium, protein conformation fluctuates, eventually visiting all possible higher-energy states in equilibrium with the ground state conformation. For soluble proteins the dynamics of such fluctuations have been successfully detected and characterized by hydrogen/deuterium exchange (HDX) combined with NMR spectroscopy. However, experimental limitations of NMR spectroscopy have precluded a similar level of understanding of the dynamics of membrane proteins. Proteins dynamics manifest themselves also when a fast perturbation is applied, such as when a laser pulse is applied to proteins with photocycles. Both in HDX and in perturbation experiments the time-resolved response of the system is a multi-exponential relaxation process. However, the relevant information to characterize and understand the protein dynamics is not the relaxation process itself, but the number, value, and nature (discrete/distributed) of the rate constants of the relaxation process. In this project our first goal is the improvement, development and application of maximum entropy and Bayesian methods to analyze the multi-exponential data arising in the HDX of membrane proteins, and in the photocycles of membrane proteins. Simultaneously, we will implement new experimental approaches of HDX to obtain dynamics information of membrane proteins by infrared spectroscopy, and apply them to at least three membrane protein systems: the melibiose transporter, bacteriorhodopsin, and a G-protein coupled receptor chimera. The combined success of both interdisciplinary goals should provide unique and fundamental information for a better understanding of membrane protein dynamics. Fields of science natural sciencesmathematicsapplied mathematicsstatistics and probabilitybayesian statisticsnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesphysical sciencesopticsspectroscopyabsorption spectroscopynatural sciencesphysical sciencesopticslaser physics Keywords Bayesian inference G-protein coupled receptors H/D exchange dynamics maximum entropy melibiose transporter membrane proteins photocycles rhodopsins time-resolved infrared spectroscopy Programme(s) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Topic(s) PEOPLE-2007-4-3.IRG - Marie Curie Action: "International Reintegration Grants" Call for proposal FP7-PEOPLE-IRG-2008 See other projects for this call Funding Scheme MC-IRG - International Re-integration Grants (IRG) Coordinator UNIVERSITAT AUTONOMA DE BARCELONA EU contribution € 50 000,00 Address EDIF A CAMPUS DE LA UAB BELLATERRA CERDANYOLA V 08193 Cerdanyola Del Valles Spain See on map Region Este Cataluña Barcelona Activity type Higher or Secondary Education Establishments Administrative Contact Esteve Padrós (Dr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data
