Objective
Highly polarized cells (pollen tubes, root hairs) are an ideal system to investigate signal transduction mechanisms in plant cells. Technical advances in genetics, molecular and cell biology made them excellent models for studying signal perception and transduction in effecting morphogenetic changes. This proposal aims to evaluate how signaling pathways previously identified modulate membrane trafficking and microfilament architecture connecting structure and function. The grant focuses on the role of SAC and syntaxin proteins in polar growing cells. Mutation of SAC1, an Arabidopsis SAC Domain Phosphoinositide Phosphatase, causes alterations in cell morphogenesis and actin organization. We have selected SAC genes highly expressed in tip-growing cells to determine how mutations in these genes affect growth and morphogenesis. We will test SAC dependency over phosphoinositide levels and how this affects the actin cytoskeleton (imaged with GFP-fusion proteins). Using imaging and molecular methods we will test SAC localization and interaction with lipid kinases and syntaxins. Should it prove positive, we will characterize it genetically, biochemically and cellularly. With these experiments we will evaluate if such signaling pathways operate independently or in cascade mode. Previous experiments have also shown that Rab GTPases are key regulators of membrane trafficking and the actin cytoskeleton, namely through modulation of lipid kinase and syntaxin activity. This has only been done independently in distinct cell models and we will test if these small GTPases are a central link of these signaling pathways.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences cell biology
- natural sciences biological sciences biochemistry biomolecules lipids
- natural sciences biological sciences genetics mutation
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2007-4-2-IIF
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
1749 016 Lisboa
Portugal
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.