Cel Transcription factors have been shown to play an important role in the tight control and for fine-tuning of lineage decisions that take place during the development of the various different cell types present in blood. Interestingly, certain factors appear to be essential for genetic programming of specific lineages, and in the absence of such factors, lineage promiscuity has been observed. We have now derived a novel mouse model system to further address the role of specific myeloid transcription factors during hematopoeisis and to determine their essential functions during macrophage specification. Interestingly, in vitro differentiation of cells derived from these animals gives rise to terminally differentiated, functional macrophage cultures with sustained self-renewing activity. Preliminary results show that in addition to myeloid-specific markers, such macrophages can upregulate some B-cell specific genes in response to M-CSF withdrawal. Whereas lymphoid to myeloid reprogramming has been well documented, the reverse has not been achieved. Here we will analyse whether macrophage to B-cell reprogramming is also possible and outline a novel experimental system to test this hypothesis both in vitro and in vivo. Knowledge gained from these experiments will add strength to a growing body of evidence supporting the role of specific transcription factors in the control of lineage reprogramming and plasticity. Słowa kluczowe Haematology Health sciences lineage macrophage transcription factor Program(-y) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Temat(-y) PEOPLE-2007-4-2.IIF - Marie Curie Action: "International Incoming Fellowships" Zaproszenie do składania wniosków FP7-PEOPLE-2007-4-2-IIF Zobacz inne projekty w ramach tego zaproszenia System finansowania MC-IIF - International Incoming Fellowships (IIF) Koordynator UNIVERSITE DE LA MEDITERRANEE D'AIX-MARSEILLE II Wkład UE € 163 643,71 Adres Boulevard Charles Livon, Jardin du Pharo 58 13284 MARSEILLE Francja Zobacz na mapie Rodzaj działalności Higher or Secondary Education Establishments Kontakt administracyjny Céline Damon (Ms.) Linki Kontakt z organizacją Opens in new window Strona internetowa Opens in new window Koszt całkowity Brak danych
