Objective
Cell function and viability are dependent upon proper protein folding. To avoid abnormal and toxic deposition of proteins, the cells recruit the ubiquitin-proteasome system, by which ubiquitin-conjugating enzymes (E2s) and ubiquitin ligases (E3s) work together to assemble poly-ubiquitin chains on misfolded or misassembled proteins, to be marked for degradation by the 26S proteasome. With the maturation of the ubiquitin research field, a number of general principles have emerged about the enzymatic mechanisms of ubiquitin ligation to proteins. In particular, several high-resolution structures of different E2 and E3 enzymes have been determined, and these have yielded many insights into the mechanisms of ubiquitin-protein attachment. However, despite these advances, the fundamental mechanism(s) of ubiquitin-chain assembly remains unknown. The textbook version- which has not been proven incorrect- holds that ubiquitin molecules are added one at a time, first to the substrate protein and then to the distal end of the growing ubiquitin chain. Conversely, several recent publications have challenged this view by showing that polyubiquitin chains can pre-form on the catalytic cysteine of Ubc7, an E2 enzyme of the protein quality control ubiquitylation system, prior to transfer into a substrate. This led to our hypothesis that polyubiquitin-Ubc7 conjugates may represent an intermediate form, en route towards substrate ubiquitylation. Our specific aims are designed to provide a comprehensive evaluation of the enzymatic steps that precede substrate ubiquitylation in protein quality control pathways, with experimental emphasis on Ubc7-mediated ubiquitin chains catalysis. To this end, it is necessary to setup both cellular and cell-free ubiquitylation systems to provide the basic tools for our research. This will be accompanied by structure-function analyzes, to identify functional elements within Ubc7, and to evaluate their mode of action.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- engineering and technology electrical engineering, electronic engineering, information engineering electronic engineering control systems
- natural sciences biological sciences biochemistry biomolecules proteins protein folding
- natural sciences chemical sciences catalysis
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
You need to log in or register to use this function
We are sorry... an unexpected error occurred during execution.
You need to be authenticated. Your session might have expired.
Thank you for your feedback. You will soon receive an email to confirm the submission. If you have selected to be notified about the reporting status, you will also be contacted when the reporting status will change.
Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2007-4-3-IRG
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
91904 JERUSALEM
Israel
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.