Objective
The aim of this proposal is to characterize the metabolic impact of the expression of key transcripts involved in fatty acid metabolism by using the model organism Caenorhabditis elegans. In particular, the main focus of the project will be on the systemic manipulation of the expression of genes for the fatty acid desaturases by an RNAi based intervention to understand their key role as regulatory control points in metabolism. To achieve this aim we will make use of state of the art metabolomic approaches including Nuclear Magnetic Resonance (NMR) spectroscopy, Gas Chromatography Mass Spectroscopy (GC-MS) and Liquid chromatography Mass Spectrometry (LC-MS) to provide a global, non-targeted description of systemic metabolism. Metabolomic analysis, conducted on different genetically modified animals, will result in a rich data-base of metabolomic profiles that, with the help of multivariate data analysis techniques, will permit the identification of specific biochemical markers that determine which pathways have been perturbed. In addition, we will also analyze flux changes directly using stable isotope labelled substrates experiments. The combination of steady state concentration data and flux changes will provide a description of the reorganization of the metabolism following the targeting of a given gene by our RNAi based intervention. Overall, the results will allow a global insight into consequences of altered genetic expression across the whole system of metabolism without any loss of intrinsic complexity - a so-called top-down systems biology approach. In addition understanding the metabolic roles the desaturases play in regulating global lipid metabolism will help us to increase our understanding of human metabolic diseases associated with lipid metabolism dysfunction including obesity, type II diabetes, dyslipidaemia and the metabolic syndrome.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences computer and information sciences data science
- natural sciences biological sciences genetics
- natural sciences biological sciences biochemistry biomolecules lipids
- natural sciences chemical sciences analytical chemistry mass spectrometry
- natural sciences physical sciences optics spectroscopy
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-IEF-2008
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
CB2 1TN CAMBRIDGE
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.