Objective
Proteins evolve to highly diverged sequence, function and structure. The dynamics of protein evolution is fundamental for understanding how natural protein evolve, and also for creating new proteins in the laboratory for medical and industrial use. “Epistasis”, which is the interaction between the effects of mutations, is often observed in nature and is the central to understand the dynamics of evolution, because it shapes the passages in the evolution. However, epistasis has been considered to be a quite complicated phenomenon and difficult to predict, thus it seemed that the dynamics of protein evolution was difficult to understand. In this project, I propose a new integrated protein evolution model which describes the fitness of protein from the physico-chemical properties of proteins. In this model, the structure of the fitness cause of epistasis of the fitness even though there is no interaction between the effects of mutations in physico-chemical properties; The fitness of protein (enzymatic activity in the cell: v0) is multiplication of function (enzymatic activity: kcat/KM) and fraction of functional expression level of protein (enzyme concentration: [E]). And the fraction of functional expression level is non-linearly correlated to the stability of proteins (∆∆G) due to unfolding, aggregation, degradation of proteins. This model will provide a new insight of epistasis and enable us to predict and understand the dynamics of protein evolution. I will validate and refine the model comparing the experimental evolution experiments. Furthermore, I will apply this model to analyze mono-genetic disease related proteins to establish the way to diagnose potential patients. This integrated protein evolution model will revolutionize the way how we study the dynamics of protein evolution, innovate the methodology of directed evolution, and make significant impact on the study of the network and biological evolution.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics mutation
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-IEF-2008
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
CB2 1TN CAMBRIDGE
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.