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Content archived on 2024-06-18

Functional analysis of the sugar based signalling process coordinating plant cell wall, primary metabolism and photosynthesis

Objective

Photosynthesis enables the plant to capture solar energy and to store it in form of sugars in the plant cell wall. Therefore plant cell walls are of particular interest in terms of optimization of energy biomass characteristics and yield. The so-called cell wall integrity mechanism monitors the state of the plant cell wall and initiates changes in its structure and composition to maintain the functional integrity. The proposed research focuses on the dissection of this mechanism by analysing the cross-talk between cell wall stress response and primary metabolism / photosynthesis. To investigate the role of sugars in the signal transduction of the cell wall stress response, I will establish the FRET-based nanosensor technique to visualise sugars in planta using Arabidopsis thaliana seedlings and determine changes in sugar concentrations in response to cellulose biosynthesis inhibition (CBI, caused by isoxaben). In addition, I will investigate the role of a cell wall invertase (ATCWINV6) in sugar signalling processes during CBI. Therefore, a detailed phenotypic characterisation of mutant plants and functional analysis of the gene / protein will be performed. To determine the effects of cell wall stress on plant metabolism I will perform detailed metabolic profiling of wildtype Arabidopsis seedlings exposed to CBI in combination with expression profiling of photosynthesis and starch metabolism genes. Furthermore, selected mutants involved in the cell wall stress response will be analysed regarding changes in sugar concentrations and metabolic effects in response to CBI. The results of this project will contribute to the understanding of the cell wall integrity mechanism that can change / regulate the quality of biomass. By shining light on the regulatory connection between cellulose biosynthesis and metabolism / photosynthesis this project will help to develop novel strategies to improve biomass quantity.

Fields of science (EuroSciVoc)

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Keywords

Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)

Topic(s)

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Call for proposal

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FP7-PEOPLE-IEF-2008
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Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

MC-IEF - Intra-European Fellowships (IEF)

Coordinator

IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE
EU contribution
€ 171 300,62
Address
SOUTH KENSINGTON CAMPUS EXHIBITION ROAD
SW7 2AZ LONDON
United Kingdom

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Region
London Inner London — West Westminster
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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