Objective
The quantitative analysis of protein mixtures is pivotal for the understanding of variations in the proteome of living systems. Therefore, several approaches have been devised in recent years, which generally allow only the relative quantitative analysis of peptides and proteins. We have presented proof of concept of a new MeCAT technique, which offers a quantitative determination of proteins and peptides. A macrocyclic metal chelate complex (DOTA) loaded with different lanthanides (Me(III)-ions) is the central part of the tag. The combination of DOTA with an affinity anchor for purification and a reactive group for reaction with amino acids (here: Cysteine) is a reagent that permits to quantify peptides and proteins in an absolute fashion. For the quantitative determination, the tagged peptides and proteins were analyzed using flow injection ICP-MS. With ICP-MS the metals can be detected with high precision, low detection limits and great dynamic range. The course of the labelling reaction has been studied using SDS-PAGE and MALDI-TOF-MS, ESI-MS and ICP-MS. To limit the width in isotopic spread and to increase the sensitivity, we used up to now the mono-isotopic lanthanides. The detection limit for Bovine Serum Albumin was calculated to 110 attomol; more examples have been analyzed. In this new project, 2D LC techniques interfaced to ESIMS and ICPMS will be combined with our metal tagging. Different LC types will be examined as 1st dimension and different 2D separation techniques as well. We assume that the detection limits for modified Proteins and peptides can be improved using the strategies similar to shotgun and Mudpit techniques; several model systems are available. In the next step, MeCAT will be applied for the analysis of bacterial proteomes and membrane proteins. In addition new concepts for antibody tagging will developed fro the specific detection of antigens in target proteins of medical significance.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- natural sciences biological sciences biochemistry biomolecules proteins proteomics
- natural sciences biological sciences biochemistry biomolecules
You need to log in or register to use this function
We are sorry... an unexpected error occurred during execution.
You need to be authenticated. Your session might have expired.
Thank you for your feedback. You will soon receive an email to confirm the submission. If you have selected to be notified about the reporting status, you will also be contacted when the reporting status will change.
Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-IEF-2008
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
10117 Berlin
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.