Objective
Only a subset of tumour cells is capable of giving rise to new tumours. These so called “tumour stem-cells” are functionally similar to adult stem cells and have been identified in tumours of several tissues. Whether transformation of adult stem cells into tumour stem cells is the event leading to the disease is not clear. Alternatively, tumours could arise from the population of committed progenitors, which have de novo acquired self-renewal properties. The aim of the proposed project is to investigate the role of Hedgehog (Hh) signalling in mammary gland stem/progenitor cell fate and cancer development. The following questions will be addressed: 1. Does induction of Gli1 expression expand the stem/progenitor cell population in the mammary gland and are the functional properties of these cells changed? 2. Does induction of Gli1 expression expand the population of Lgr5+ cells in Gli expressing mammary glands and Gli1 induced tumours? 3. Does induction of Gli1 expression result in additional genetic changes relevant for tumour formation? 4. Does induction of Hh-signalling in Lgr5+ mammary cells cause tumour formation? 5. Would prevention of Gli activation with small molecule inhibitors like GANT61 prevent mammary cancer formation? The activity of the Hh pathway is required for growth of a variety of tumours, including breast cancer. We propose to use tetracycline-controlled conditional expression of Gli1, the main effector of the Hh-signalling pathway, in mouse mammary gland as a model system. The dynamic changes in the stem/progenitor cell population will be monitored after induction of Gli1 expression. Stem cells will be sorted based on known markers, including the orphan G protein coupled receptor Lgr5. The role of Hh signalling in Lgr5+ cells and the tumorigenecity of Lgr5+ cells will be addressed. This project will contribute to our understanding of breast cancer development and have implications on future therapeutic strategies of mammary cancer.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- natural sciences biological sciences biochemistry biomolecules proteins
- medical and health sciences medical biotechnology cells technologies stem cells
- medical and health sciences clinical medicine oncology breast cancer
You need to log in or register to use this function
We are sorry... an unexpected error occurred during execution.
You need to be authenticated. Your session might have expired.
Thank you for your feedback. You will soon receive an email to confirm the submission. If you have selected to be notified about the reporting status, you will also be contacted when the reporting status will change.
Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-IEF-2008
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
171 77 STOCKHOLM
Sweden
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.