Objective
During the last few years, single-molecule fluorescence (SMF) methodologies have revolutionized the accessible information on biomolecular complexes, molecular dynamics, and biological mechanisms. Novel and important structural and dynamic insights can be obtained by studying molecules one by one, particularly into biological processes. In this field, the development of new methodologies and tools, on one hand, and of new improved fluorophores, on the other, are two major lines of research. In the current proposal, a cutting edge SMF methodology will be implemented: SMF Pulsed Interleaved Excitation (PIE) with two-colour coincidence detection, a technique that allows alternating excitation of fluorophores of different colours acting as donor and acceptor in fluorescence resonance energy transfer (FRET) processes. SMF-PIE has several advantages over the conventional SMF schemes to study biological systems, such as accurate determination of FRET efficiencies and inter-fluorophore distances, effective removal of the spectral, or distinction between molecules that have a low FRET efficiency from those that lack acceptor tag. The instrument performance will be calibrated and validated using model samples. Subsequently, SMF-PIE will be applied in three multidisciplinary biophysical approaches: 1) testing the feasibility and performance of newly synthesised fluorescein derivatives for SMF studies; 2) the resonance energy transfer between the new dyes acting as FRET donors and a DNA intercalator fluorophore as acceptor will provide insights into the mechanisms of intercalation of these carcinogenic molecules; 3) SMF-PIE will be employed to study the early pre-fibrillar aggregates from an amyloidogenic protein, the SH3 domain of α-spectrin, providing insights into the amyloid fibril formation mechanisms, a major biophysical and biomedical challenge nowadays.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- engineering and technology materials engineering colors
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences biochemistry biomolecules proteins
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-ERG-2008
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
18071 GRANADA
Spain
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.