Objective
The gold standard for obtaining data on ion channel function is the patch clamp method. The patch clamp technique is very labor intensive and has ultra low data throughput which has been highly motivating for the development of automated patch clamp systems.
Nanion has developed a technology for performing automated patch clamp recordings on planar glass substrates, with comparable recording quality as with conventional patch clamping, however, lacking the possibility to very briefly expose the cells to different solutions. If exposure times are too long, this can have adverse effects when investigating ion channels that activate on the addition of agonists (ligand gated ion channels). Ligand gated ion channels are gaining more attention in research and drug development because of their involvement in disease. These ion channels often display desensitization, resulting in a diminishing compound response, during constant stimulation. Recovery kinetics often depends on exposure time, thus are brief exposure times desirable.
We aim at developing a method for very short compound exposure using automated patch clamp by combining microfluidic structures for liquid administration to the patch clamp substrate. A robotic pipettor arm applies all solutions to the microchannels and by subsequently aspirating two differnt solutions of buffer into the pipette, and immediately adding this “stack“ of solutions to the cell, the cell experiences a very brief exposure of agonist, directly followed by wash buffer. This way, exposure times are anticipated to be shorter than 1 second, with switching times in the order of 50 ms.
This would allow for accurate experiments on ligand gated ion channels that exhibit rapid desensitization , which in turn would be useful in ion channel research on channelopathies, and to facilitate the drug development of compounds modulating ligand gated ion channels.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences basic medicine pharmacology and pharmacy drug discovery
- natural sciences biological sciences biochemistry biomolecules proteins
- engineering and technology materials engineering
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-ERG-2008
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
80339 Munchen
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.