Objective
Synaptogenesis and synaptic plasticity rely on protein synthesis, in particular on localized translation within dendrites. The aim of my project will focus on investigating the multiple and overlapping molecular mechanisms that contribute to fine-tuning the expression of dendritic mRNA at synapses. In my previous work, I have identified a novel regulatory pathway that involves splicing and modulates the stability and expression of a subset of neuronal and dendritic mRNAs. Prominent amongst the mRNAs analyzed in my studies is Arc, encoding a protein essential for memory consolidation and maintenance of LTP. Several approaches will be undertaken to elucidate the molecular elements controlling Arc mRNA expression: 1) In vivo biochemical isolation and characterization of the ribonucleoprotein particle associated with Arc mRNA 3’UTR region. The procedure will be designed in order to identify trans-acting factors whose binding is connected to: i) synaptic activity ii) dendritic transport iii) splicing of the mRNA. 2) To unveil the role of the identified factors, assays will be carried out in vivo and in cultured neurons, and will include: silencing via lentiviral expression of siRNAs, microscopy, electrophysiology and expression of reporter Arc genes carrying specific modifications. 3) Involvement of miRNAs and of cis-acting elements in controlling synaptic Arc protein expression will also be tested. miRNAs-dependent regulation will be verified in cultured neurons and potential coupling with other pathways of translational and mRNA stability regulation will be tested. Overall, the in vivo biochemical and functional characterization of both cis- and trans-acting factors controlling the expression of Arc mRNA, will allow a better understanding of the mechanisms underlying its modulation, and likely shed light on the pathways controlling dendritic mRNA localized expression and synaptic plasticity.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences physical sciences optics microscopy
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-IRG-2008
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
00161 Roma
Italy
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.