Objective
After DNA damage a signal must be created to recruit DNA repair factors to the lesion. Then the repair machinery needs to gain access to DNA and chromatin should be properly reassembled. DNA assembly with histones to form chromatin creates a physically limits the access to DNA. Alterations of chromatin that do not affect the DNA sequence constitute the epigenetic level of regulation of DNA metabolism. Among them, the post-translational modification of histones can directly regulate chromatin structure and they can also recruit different proteins to DNA serving as signaling and docking points. There is not much information regarding the role of these modifications in DNA damage response (DDR). This project looks forward to gaining insight into the role of histone H3K79 and histone H4K20 methylation in recuiting DNA repair factors. 53BP1 has been proposed to be recruited to the lesions by methylation of H3K79 and/or H4K20, although the mechanism is not still clear. In yeast, Dot1, the enzyme responsible for H3K79 methylation, is required for a normal DDR. As a second objective we would like to analyze the potential modifications of H3K56 in mammalian cells and determine if they have a function in DDR. Also in yeast, it has been described that H3K56 acetylation is necessary for the re-assembly of chromatin after repair. In mammals it seems that methylation of this residue takes place instead of acetylation. However the role of these marks has not been well studied in mammals. We will employ in vitro cell culture to analyze the exposure of these marks after DNA damage, the changes in localization or protein interactions of the enzymes responsible for them and to identify proteins that create or recognize these marks. Additionally KO mice will be used to analyze the physiological relevance of the results in vivo. All these studies are highly relevant to the EU, as they imply the interconnection of two priority fields in EU (both of them have a European Network).
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences zoology mammalogy
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-IOF-2008
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
28029 Madrid
Spain
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.