Objective
The heart is the first organ to form and it is essential for blood distribution during embryogenesis. Congenital heart malformations are the most common birth defect in humans, present in 1 % of the human population worldwide.
The calcineurin splicing variant CnAbeta1 is expressed in stem cells, precursors and developing tissues and its expression decreases during tissue maturation. We aim to determine the role of CnAbeta1 during different stages of embryonic development.
Aims:
1. Identify the role of CnAbeta1 in embryonic stem (ES) cell biology:
We will knock down CnAbeta1 expression in ES cells using siRNA constructs and study their self-renewal and differentiation capacity.
2. Study embryonic development of transgenic mice in which CnAbeta1 expression is reduced ubiquitously:
We will generate transgenic mice in which CnAbeta1 expression will be knocked down ubiquitously by overexpression of a specific CnAbeta1 shRNA. We will identify morphological defects and study target tissues using immunohistochemical and biochemical approaches.
3. Determine the role of CnAbeta1 in cardiac development by using conditional knockdown mice in which CnAbeta1 expression is reduced specifically in the heart:
We will overexpress CnAbeta1 shRNA specifically in the heart from E8.0 onwards by using Cre/Lox technology. We will study the effects of CnAbeta1 knockdown by immunostaining and biochemical approaches and study cardiac function in the adult heart by using echocardiography and telemetry. Transcriptional and phosphoprotein profiles will be analyzed using DNA and protein microarrays.
4. Study the role of CnAbeta1 in the epicardium during embryonic development and in adult heart:
We will overexpress CnAbeta1 shRNA specifically in the epicardium using Cre/Lox technology. Mice will be analyzed as in Aim 3. We will study the ability of epicardium-derived cells (EPDC) to migrate and undergo EMT in the absence of CnAbeta1.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences cell biology
- natural sciences biological sciences developmental biology
- medical and health sciences medical biotechnology cells technologies stem cells
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-ERG-2008
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
28029 Madrid
Spain
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.