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Content archived on 2024-05-27

In vivo choreography of DNA molecules and repair proteins during the search for homology

Objective

Double-strand breaks (DSB) constantly challenge the integrity of the cellular genome and are important contributors to cancer and genetic diseases. Repair of damaged DNA is a dynamic process that requires careful orchestration of a multitude of enzymes and chromatin constituents that are organized into centers (foci). The outgoing laboratory recently developed powerful methods to explore the dynamics of focus assembly/disassembly in vivo. Using these techniques, I will explore one of the most intriguing steps of homologous recombination: the search for homology. During homologous recombination, the broken DNA searches for intact homologous dsDNA within the entire genome. To elucidate when and how the process of homology search occurs in vivo, I will visualise the movement of a donor and an acceptor sequence, as well as recombination factors, before, during and after double strand break repair. I will take advantage of the elegant yeast genetic system and explore the effects of targeted mutations in key enzymes involved in this process. In the return laboratory, I will to transpose the assay developed in the outgoing laboratory in mammalian cells. With the aid of the Marie Curie fellowship, I propose to spend my post-doctoral period to evolve from single molecule approaches in vitro to genetics and in vivo systems involving multiprotein complexes.

Fields of science (EuroSciVoc)

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Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

FP7-PEOPLE-IOF-2008
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Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

MC-IOF - International Outgoing Fellowships (IOF)

Coordinator

ECOLE NORMALE SUPERIEURE
EU contribution
€ 242 632,79
Address
45, RUE D'ULM
75230 Paris
France

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Region
Ile-de-France Ile-de-France Paris
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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