Dissecting the new “platform-binding site” on the bacterial ribosome. Role of particular ribosomal proteins in regulating translation initiation in bacteria

Project Information

PBS

Grant agreement ID: 237621

Project closed

Start date 1 February 2010

End date 31 January 2012

Funded under

• Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)

Total cost No data

EU contribution € 172 267,71

Coordinated by

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS
France

Objective

Recent X-ray crystallographic and cryo-electron microscopy data indicate that structured mRNAs bind to the bacterial ribosome at an early step of translation initiation, to a newly characterised ribosomal site located at the “platform” of the small ribosomal subunit (the “platform-binding” site or PBS). In a second step, the mRNAs are supposed to unfold and to migrate to the P-site to interact with the initiator tRNA. The proposal aims at dissecting the role of ribosomal proteins situated at the PBS and potentially contacting the structured 5’-terminal parts of mRNAs. It is proposed to isolate mutants in the genes of the corresponding proteins that influence the in vivo translation of the lacZ gene fused to different model 5’-UTRs known to be involved with translation regulation. Once such mutations are found, global analysis of their effect will be performed and their effect will be analyzed in vitro.

Fields of science (EuroSciVoc)

CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.

• natural sciences › biological sciences › microbiology › bacteriology
• natural sciences › biological sciences › biochemistry › biomolecules › proteins
• natural sciences › physical sciences › optics › microscopy
• natural sciences › biological sciences › genetics › mutation

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Programme(s)

• FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)

Topic(s)

• FP7-PEOPLE-IIF-2008 - Marie Curie Action: "International Incoming Fellowships"

Call for proposal

FP7-PEOPLE-IIF-2008
See other projects for this call

Funding Scheme

MC-IIF - International Incoming Fellowships (IIF)

Coordinator

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS

• EU contribution: € 172 267,71
• Address: RUE MICHEL ANGE 3 ; 75794 Paris ; France
• Region: Ile-de-France › Ile-de-France › Hauts-de-Seine
• Activity type: Research Organisations
• Total cost: No data