Objective
A major goal of biology is to explain how gene regulatory information is encoded by the genome. To date, we cannot decipher this regulatory code, despite the cells ability to read it in natural and artificial sequence contexts, and in contrast to our detailed understanding of the genetic code, which allows us to seamlessly translate DNA into protein sequences. Here, I propose a regulatory genomics approach in Drosophila with three specific objectives: First, we will determine the sequence basis of how individual Hox transcription factors (Scr, Antp, Ubx, abd-A, Abd-B) and factors downstream of signalling pathways (Sd, pan, ci, Su(H), pnt, Stat93E, Mad, Smox, CrebA) regulate different genes in different tissues. We will perform tissue-specific ChIP-Seq and measure gene expression in mesoderm/muscle, epidermis, and neurons, and explain common and tissue-specific targets by their sequences. Second, we will determine requirements for enhancer function in several different cell-types, by performing an exhaustive and unbiased enhancer screen and computationally analyzing the sequences. Third, we will build a computational model to extract general rules from objectives 1 & 2, learn the regulatory codes, and make specific predictions. We will validate our model using cross-validation and predictions with unrelated sequence (e.g. from yeast), and will finally use it to design enhancers that are active in specific cell-types or combinations of cell-types. Our combination of experimental and computational methods makes us confident that we will make major contributions to the understanding of gene regulation in the fly. We anticipate that we will learn general principles, which will hold across tissues and animals, and might ultimately lead to the general regulatory code.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences computer and information sciences computational science
- natural sciences biological sciences genetics genomes
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
ERC-2009-StG
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Host institution
1030 Wien
Austria
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.