Objective The control of DNA based processes in biology is crucial for an organism s life, development, reproduction and evolution. In fact, deregulation of gene expression, of the replication program, of DNA repair or recombination has often disastrous consequences. The eukaryotic genome is structurally and functionally organized into chromatin by a highly complex mixture of proteins that control access to the DNA. In particular, heterochromatin proteins play a prominent role in this control. Genetics, biochemistry and molecular biology identified many players involved in eukaryotic DNA biology and provided everything we currently know about it. However the full composition of a given locus remains largely obscure and just like any object under scientific study, knowing the composition is an absolute pre-requisite for a full understanding of its features. Consequently, the nature of the interactions between chromatin structural components and DNA machineries is poorly understood and the elaboration of models from rather incompletely characterized systems can be misleading. During my post-doctorate, I have developed an unbiased approach for the in vivo purification of chromatin proteins in a locus specific manner, and I intend to apply this new technology in combination with a genetic approach. Monitoring the consequence of heterochromatin protein loss on the full composition of regulated loci will allow a deeper understanding of the role these proteins in the regulation of eukaryotic genome biology. Fields of science natural sciencesbiological sciencesgeneticsDNAnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymesnatural sciencesbiological sciencesmolecular biologynatural sciencesbiological sciencesgeneticsgenomeseukaryotic genomes Programme(s) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Topic(s) ERC-SG-LS2 - ERC Starting Grant - Genetics,Genomics,Bioinformatics and Systems Biology Call for proposal ERC-2009-StG See other projects for this call Funding Scheme ERC-SG - ERC Starting Grant Host institution INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE EU contribution € 1 707 500,00 Address RUE DE TOLBIAC 101 75654 Paris France See on map Region Ile-de-France Ile-de-France Paris Activity type Research Organisations Administrative Contact Vincent Boyer (Mr.) Principal investigator Jerome Dejardin (Mr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data Beneficiaries (1) Sort alphabetically Sort by EU Contribution Expand all Collapse all INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE France EU contribution € 1 707 500,00 Address RUE DE TOLBIAC 101 75654 Paris See on map Region Ile-de-France Ile-de-France Paris Activity type Research Organisations Administrative Contact Vincent Boyer (Mr.) Principal investigator Jerome Dejardin (Mr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data
