Objective
While automation is revolutionizing many aspects of biology, the determination of three-dimensional protein structure remains a long, hard, and expensive task. Novel algorithms and computational methods in biomolecular NMR are necessary to apply modern techniques such as structure-based drug design on a much larger scale. The goal of this project is to address a key computational bottleneck in NMR structural biology, resonance assignments. We will accelerate protein NMR assignment by exploiting a priori structural information. By analogy, in X-ray crystallography, the molecular replacement (MR) technique allows solution of the crystallographic phase problem when a “close” or homologous structural model is known, thereby facilitating rapid structure determination. In contrast, a key bottleneck in NMR structural biology is the assignment problem. An automated procedure for rapidly determining NMR assignments given an homologous structure, will similarly accelerate structure determination. Moreover, even when the structure has already been determined by crystallography or computational homology modeling, NMR assignments are valuable because NMR can be used to probe protein-protein interactions and protein-ligand binding (e.g. via chemical shift mapping), and dynamics (via, e.g. nuclear spin relaxation). We will develop an MR-like approach for structure-based assignment of resonances and NOEs, to be applied when a homologous protein is known. The tool that we develop will accept both CH- and NH- RDCs, and 4-D NOESY data, and will implement a Bayesian scoring function for structure-based assignments. It will provide the user the option to use only NH RDCs or NH and CH RDCs and will be tested on real proteins. The source code will be released as open source with the user manual.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- natural sciences biological sciences biochemistry biomolecules proteins proteomics
- natural sciences earth and related environmental sciences geology mineralogy crystallography
- natural sciences computer and information sciences computational science
- natural sciences mathematics pure mathematics topology computational topology
- natural sciences biological sciences molecular biology structural biology
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2009-RG
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
34956 Istanbul
Türkiye
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.