Objective
This project aims to unravel the molecular architecture of the structural components of the presynaptic terminal of hippocampal neurons grown in culture and to elucidate their functional properties. These are closely related to the synaptic vesicle release, one of the central questions in synaptic function, as well as with the development and maturation of synapses. The investigation of the structural relations among synaptic vesicles and between synaptic vesicles as well as other membranous and cytoskeletal structures at the presynaptic terminals are of consequence for the apprehension of neurotransmitter pools and will eventually help explain the neuronal behaviour in physiological stimulus experiments. More specifically, the project sets out to elucidate the role of large dense-core vesicles in the transport of the presynaptic scaffolding protein Bassoon Packets of dense core vesicles, together with vesicular and tubulovesicular structures, are thought to transport presynaptic scaffolding molecules such as Bassoon and Piccolo, as well as other proteins needed for the assembly of presynaptic terminals. Although these packets are mostly mobile, they can be stabilized when a stable contact with a dendrite is formed, presumably forming a nascent synapse. It has been proposed that such as the fusions of dense core vesicles with the presynaptic plasma membrane are required for the development of functional presynaptic terminals from these contacts. To achieve these objectives we are to use a cryo-electron tomography (cryo-ET) approach. Prior to the knowledge acquisition, the conditions and developing methods needed to detect synapses suitable for cryo-ET investigations in an efficient and reproducible manner must be established.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences physical sciences optics microscopy electron microscopy
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2009-IEF
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
80539 MUNCHEN
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.