Objective
The genomes of pathogenic bacteria often contain multiple copies of genes with similar sequence. The encoded proteins are generally secreted or associated with the bacterial surface and are thus likely to be involved in the host-pathogen interaction. However, the functions of these multigene families in pathogenesis remain largely unknown. We will investigate the role of bacterial multigene family proteins in the host-pathogen interaction taking a Systems Biology-based approach. We will focus on two model pathogen-specific virulence-related proteins families: the Inl (internalin) proteins from the gram-positive genus Listeria, and the Vap (virulence-associated) proteins from Rhodococcus equi, a pathogenic actinomycete related to Mycobacterium tuberculosis (MTB). We will also investigate the Mce and PE/PPE proteins, which are distributed among several pathogenic actinobacteria including R. equi and MTB. Different members of these multigene families are found in genotypes or species differing in virulence and pathogenic tropism. Our hypothesis is that the specific multigene complements carried by the different “pathovars” contribute to their distinct pathogenic properties. We will address the research question by systematically identifying binding partners to the investigated proteins and relevant domains thereof by interaction proteomics using high-throughput yeast two-hybrid (Y2H) screens with large host (mammalian) cDNA prey libraries. We will then apply bioinformatics tools and network analysis to define the host subcellular compartments and functional networks and pathways targeted by the bacterial multigene family proteins, and the global contribution of the identified interactions to pathogenesis. Microbial surface-associated and excreted proteins are at the forefront of the host-pathogen interplay, mediating adherence/colonisation, subverting host pathways, or modulating innate or acquired immunity.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins proteomics
- natural sciences biological sciences microbiology bacteriology
- natural sciences biological sciences genetics genomes
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2009-IEF
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
EH8 9YL Edinburgh
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.