Objective
Cancer diseases affect millions of people worldwide and are a source of great burden to society, both financially and in terms of health care resources. In Europe, Every day 5.214 Europeans are diagnosed with cancer and 3.185 die from their disease (20% of total cases are related to lung cancer). Understanding of how genetic factors contribute to these diseases is gathering speed. Genome-wide association analyses on large patient cohorts are generating large sets of candidate genes. A frequent genetic alteration observed in heavy smokers, lung cancer and other tissue carcinomas is the deletion of chromosomal region 3p21.3 where the LUCA15/RBM5 (RNA binding Motif 5) gene maps. RBM5 protein levels are misregulated in several cancers such as lung and breast cancers and constitute one molecular signature associated with metastasis in various solid tumors. RBM5 is an RNA binding protein harboring several domains involved in RNA metabolism and pre-mRNA splicing. Recent data revealed that RBM5 binds to a U/C-rich sequence in the caspase-2 pre-mRNA and that it regulates the alternative splicing of apoptosis-related genes such as the “death receptor” Fas (CD95/APO-1) and its key inhibitor c-FLIP pre-mRNAs. The goal of this proposal is to identify and characterize of RBM5 containing complexes. We will use the Cross Link ImmunoPrecipitation (CLIP) technique coupled to deep sequencing to identify RBM5 RNA targets and to draw a functional map correlating their positions with the alternative splicing event of their targets. To identify RBM5 protein partners, we will use gel chromatography to purify RBM5 containing complex(es) and MALDI-TOFF to identify RBM5 interactors in these complex(es). This information will help us to analyze the regulation of c-FLIP pre-mRNA splicing by RBM5. Hopefully, the results will allow us the design of new potential therapeutic strategies aimed to inhibiting the massive cell transformation occurring during the tumor progression processes.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences clinical medicine oncology lung cancer
- natural sciences biological sciences biochemistry biomolecules proteins
- medical and health sciences clinical medicine oncology breast cancer
- natural sciences biological sciences genetics RNA
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2009-IEF
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
08003 Barcelona
Spain
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.