Objective
Oxidation of DNA bases by reactive oxygen species (ROS) leads to mutations that have been linked with cancer and neurodegenerations. The occurrence of these major diseases increases upon organism aging. The life expectancy in western society and particularly EU has considerably increased in the XXth century. Thus mortality caused by cancer and neurodegenerations has become massive. Therefore fighting against aging of the population is our future medical challenge and understanding mechanisms reducing the impact of oxidative DNA damages can be one of the best weapons to win this non-violent war. Mouse mutants for Nucleotide Excision Repair (NER) revealed an unexpected link between DNA damages and premature aging. The UV lesions have been for historical reason the “model lesion” for NER. However the phenotype in patients and in the several mice model of NER deficiencies together with the Oxygen Radical theory of aging suggests that endogenously generated oxidative DNA damages could be at the roots of the premature and natural aging. The molecular mechanism of NER of UV-lesions has been extensively studied but the NER of oxidative DNA lesion has not been of major focus. Our goal is to molecularly dissect the involvement of NER proteins, in the repair of oxidative DNA damages in vivo. To understand a basic process of cell physiology like mechanisms of oxidative DNA repair we will use a multidisciplinary approach with techniques spreading from mass spectrometry to live cell imaging. Our first aim would be to evaluate the recruitment of NER factors to oxidative DNA lesions generated in living cells by using confocal microscopy and a laser irradiation generating specifically those lesions. We will investigate, by mass spectrometry, for the best candidate the partners recruited during oxidative DNA repair. How DNA repair of oxidative DNA lesions occurs in a chromatin context will also be investigated by an innovative experimental design.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- natural sciences biological sciences neurobiology
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences genetics nucleotides
- natural sciences physical sciences optics microscopy confocal microscopy
- natural sciences chemical sciences analytical chemistry mass spectrometry
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2009-IEF
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
3015 GD Rotterdam
Netherlands
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.