Objective Botulinum neurotoxins (BoNTs), the most toxic substances known, are susceptible for use as bioweapons (listed as class A agents by CDC). Currently licensed animal derived antibodies or F(ab’)2 preparations, are at a high risk of inducing adverse effects and their privately-owned stockpiles are limited. In this project, we will target the most lethal types of BoNTs: A (subtypes A1 and A2), B (B1 and B2) and E (E1). The antibodies will be directed against the C-terminus of the heavy chain and the light chain of each of these three BoNTs, as these domains contain neutralizing epitopes, according to the latest scientific data. The six corresponding immunogens will be produced in recombinant form, and utilized to immunize macaques (Macaca fascicularis), from which phage-displayed immune libraries will be built. Utilizing the phage technology, scFvs cross- reacting with A1 and A2, or B1 and B2subtypes will be panned. The best scFv from each library will be selected according to its high affinity and in vitro neutralization property. The six most neutralizing scFvs will then be super-humanized (“germline-humanized”) and expressed as IgGs, which will be tested in vivo, in a standardised model of protection and against toxins obtained from collections of clostridia strains. The project includes representatives of medical first-responders who will disseminate our results, and help create a market so that the necessary clinical studies could be performed in future. The project will offer an unequalled level of security against biothreats in Europe, based upon a family of well-tolerated and effective molecules. Fields of science natural sciencesbiological sciencesmicrobiologyvirology Programme(s) FP7-SECURITY - Specific Programme "Cooperation": Security Topic(s) SEC-2009-4.3-01 - Neutralisation of CBRN effects following a terrorist event Call for proposal FP7-SEC-2009-1 See other projects for this call Funding Scheme CP - Collaborative project (generic) Coordinator MINISTERE DES ARMEES EU contribution € 136 000,00 Address RUE SAINT DOMINIQUE 14 75007 Paris France See on map Region Ile-de-France Ile-de-France Paris Activity type Public bodies (excluding Research Organisations and Secondary or Higher Education Establishments) Administrative Contact Jean-Nicolas Tournier (Prof.) Links Contact the organisation Opens in new window Total cost No data Participants (9) Sort alphabetically Sort by EU Contribution Expand all Collapse all Centre de Recherche du Service de Santé des Armées France EU contribution € 549 500,00 Address Avenue des Maquis du Grésivaudan 24 38702 La Tronche See on map Activity type Public bodies (excluding Research Organisations and Secondary or Higher Education Establishments) Administrative Contact Thibaut Pelat (Dr.) Links Contact the organisation Opens in new window Total cost No data TECHNISCHE UNIVERSITAET BRAUNSCHWEIG Germany EU contribution € 418 360,00 Address UNIVERSITAETSPLATZ 2 38106 Braunschweig See on map Region Niedersachsen Braunschweig Braunschweig, Kreisfreie Stadt Activity type Higher or Secondary Education Establishments Administrative Contact Michael Hust (Dr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data INSTITUT PASTEUR France EU contribution € 597 800,00 Address RUE DU DOCTEUR ROUX 25-28 75724 Paris See on map Region Ile-de-France Ile-de-France Paris Activity type Research Organisations Administrative Contact Thierry Planchenault (Dr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data HEALTH PROTECTION AGENCY HPA Participation ended United Kingdom EU contribution € 314 190,60 Address BUCKINGHAM PALACE ROAD 151 SW1W 9SZ London See on map Activity type Research Organisations Administrative Contact Deborah Smith (Ms.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS France EU contribution € 139 440,00 Address RUE MICHEL ANGE 3 75794 Paris See on map Region Ile-de-France Ile-de-France Paris Activity type Research Organisations Administrative Contact Michele Saumon (Ms.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data LFB-BIOTECHNOLOGIES France EU contribution € 133 081,00 Address AVENUE DES TROPIQUES, ZA DE COURTABOEUF 3 91940 LES ULIS See on map Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Administrative Contact Jacques Perron (Mr.) Links Contact the organisation Opens in new window Total cost No data HELSINGIN YLIOPISTO Finland EU contribution € 250 069,60 Address YLIOPISTONKATU 3 00014 Helsingin Yliopisto See on map Region Manner-Suomi Helsinki-Uusimaa Helsinki-Uusimaa Activity type Higher or Secondary Education Establishments Administrative Contact Sanna-Maija Miettinen (Dr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data ABSISKEY CP France EU contribution € 152 500,00 Address RUE COLONEL DUMONT 26 38000 GRENOBLE See on map Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Administrative Contact Olivier De Bardonneche (Mr.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data Medicines and Healthcare products Regulatory Agency United Kingdom EU contribution € 275 444,80 Address Buckingham Palace Road SW1W 9SZ London See on map Administrative Contact Amanda King (Ms.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data
