Objective
Cells growing in identical environments can display substantial phenotypic heterogeneity within a population. This is partly due to changes in genetic information that in turn cause systematic changes in expression rates. It has also recently been shown that even genetically identical cells behave differently because many cellular processes involve molecules present in small numbers which causes significant cell-to-cell fluctuations. Genetic and non-genetic sources of heterogeneity may be connected through fluctuations in DNA repair enzymes such as the RecBCD protein of Escherichia coli and its functional analogue AddAB in Bacillus subtilis. RecBCD/AddAB is essential for DNA double-strand breaks repair and stimulates genetic exchanges. It is expressed at low levels, thus raising the question of how bacterial cells cope with potentially large cell-to-cell fluctuations in this complex. The objective of the project is to characterize the single cell heterogeneity of DNA repair in bacteria and to evaluate its impact on cell survival. We will (i) quantify fluctuations in RecBCD protein. (ii) Manipulate fluctuation levels to measure their impact on cell survival. (iii) Interface the experimental analysis with mathematical modelling to gain a better understanding of the system. (iv) Test the generality of the system by applying the above analysis to AddAB in B. subtilis. The results will shed light on a crucial cell process where fluctuations are expected to be substantial for physiological reasons, and of great selective consequence. The project will be developed the laboratories of Prof. Paulsson (Systems Biology Department, Harvard Medical School, Boston USA) and Dr. Noirot (MICALIS institute, INRA, Jouy en Josas, France). It will give the opportunity to the applicant to be trained in a world leading group and to come back to develop her independent group in a major Systems Biology research laboratory in Europe.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences microbiology bacteriology
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
- natural sciences mathematics applied mathematics mathematical model
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2009-IOF
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
EH8 9YL Edinburgh
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.