Objective
DNA double strand breaks (DSBs) and telomeres both represent ends of a linear chromosome, and both can lead to dangerous genomic rearrangements. Recent studies in budding yeast suggest that persistent DSBs associate with factors in two perinuclear compartments, at nuclear pores and in the inner nuclear membrane (INM). The stability of short telomeres also involves telomere binding to an INM protein, Mps3, and unequal sister chromatid exchange in the rDNA array is prevented by binding another INM protein, Heh1. In the absence of this anchor, rDNA rings are excised leading to premature senescence. From these studies it is clear that the interaction of chromatin with the nuclear periphery contributes to genome stability, yet many questions remain concerning the relocalization and its functional consequences. Are these interactions part of a common mechanism to stabilize or process breaks? Do they cross-talk with each other or do the anchorage sites provide distinct activities specific to the context of the DSB? This proposal will examine the role of nuclear pore and INM proteins in telomere maintenance, rDNA stability and DSB repair, but comparing the outcome of damage at the three sites in various mutant strains. I will use high resolution fluorescence imaging to observe the movement of a randomly distributed DSB to the INM or to pores, which can be distinguished by use of fluorescent tagging and a nup133 mutation. I will determine which repair pathways are regulated by the pores and by INM factors involved in perinuclear anchoring, and finally will investigate whether common mechanisms regulate DSBs processing and the repression of recombination at telomeres or within the rDNA. Intruiguingly all of the nuclear envelope components implicated in this process have homologues in higher eukaryotic cells, which suggests that these mechanisms may be conserved.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences genetics mutation
- natural sciences biological sciences genetics chromosomes
- natural sciences biological sciences genetics genomes
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2009-IIF
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
4058 BASEL
Switzerland
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.