Objective
In previous work performed by Dr. Karatzas it has been demonstrated that the presence of glutamate does not confer any acid resistance to L. monocytogenes grown in DM. This is due to the absence of any GABA export in this medium even if glutamate is present. Furthermore, L. monocytogenes accumulates high levels of intracellular GABA when challenged with HCl in both rich media like BHI and DM. This accumulation of GABA intracellularly suggests that this response might protect cells under acidic conditions. We propose that the accumulation of intracellular but also of extracellular GABA buffers the acidic envinronment and therefore protects the cells. This hypothesis will be tested and the role of intracellular GABA will be assessed with experiments performed in a mutant missing all the decarboxylases. Furthermore, it is proposed to identify the source of intracellular GABA which is probably intracellular glutamate. In addition it will be investigated if any of the three decarboxylases is responsible specifically for the production of intracellular GABA. In previous work performed by Dr. Karatzas it has been demonstrated that previously unknown components in BHI activate the expression and the function of the GAD system in terms of GABA export. We propose to specifically identify these components and investigate if the regulation is at transcription level or if they are required for the activity of the antiporter-based GAD system.
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2010-RG
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
RG6 6AH Reading
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.