Objective
Despite its fundamental importance, our understanding of the immediate mechanisms directing tissue response and the complex cell-cell communications regulating its resolution remain fairly limited. Moreover, current anti-inflammatory drugs work relatively unspecific as on-off switches, thereby exhibiting considerable side effects. Studies on innate immunity have benefitted from the introduction of the transparent zebrafish larvae as an assay system. Transgenic fish expressing fluorescent proteins in leukocytes allow direct quantification of the inflammatory response in real-time in a live animal. However, the assays used to date require physical wounding of the fish preventing even a medium throughput experimental scale. We have developed a novel wounding assay without the need for mechanical wounding allowing high throughput large-scale analyses towards a comprehensive understanding of inflammation.
Here, I propose the generation of an automated screening platform based on our specific ‘non-invasive’ chemical wounding followed by automated microscopy and image acquisition that will allow the in vivo screening of thousands of compounds in a matter of days with respect to their effect on leukocyte recruitment, leukocyte function, resolution of the response and general toxicity in a single assay; target profiling of small molecule ‘hits’ from by chemical proteomics and integration of acquired data into an interactive web-based repository aiming at an interconnected view of molecular networks guiding an innate immune response.
This platform will allow chemical and genetic screens to the identification of new molecular pathways involved in innate immunity. At the same time, our approach will identify lead compounds for the development of novel immuno-modulatory drugs with enhanced specificity allowing fine-tuning of inflammation by targeting particular aspects of an innate immune response.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- natural sciences biological sciences biochemistry biomolecules proteins proteomics
- natural sciences physical sciences optics microscopy
- medical and health sciences basic medicine immunology
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2010-RG
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
76131 Karlsruhe
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.