Objective
Ixodes ricinus abundance and its infection prevalence with Borrelia burgdorferi s.l. (the Lyme disease agent) have led to an increased risk of human exposure to tick bites and Borrelia infection in Europe. Acaricide based control of this vector has caused serious repercussions, thus raising the importance for the development of anti-tick vaccines. My previous work has demonstrated that guinea pigs vaccinated with a salivary cystatin of Ixodes scapularis, the Lyme vector in the USA, are partially protected against tick blood feeding and that the same protein facilitates Borrelia transmission as well. I. ricinus cystatin ortho-/homologues are not studied yet, partially due to the limited genomic information for this tick. Hence, this study aims to enrich our knowledge of I. ricinus salivary transcripts by considering the entire complex tick lifecycle and by performing massive 454 pyrosequencing, a relatively novel, highly efficient and cost effective technology. In parallel, library screens will be carried out to identify I. ricinus secreted cystatins. Their tissue specificity and abundance will be estimated, while gene silencing will evaluate their contribution to tick feeding success. Recombinant cystatins will be 1) biochemically characterised for their inhibitory effect on different cysteine proteases and 2) tested for their role in vertebrate host immunomodulation/pathogen transmission. Next, vaccination studies will investigate their potential to provide protection against I. ricinus and/or Lyme disease transmission. Overall, the proposed project is expected to enhance my collaboration with the scientific community in the United States and Europe, thus promoting the transfer of knowledge and technology in the field of tick functional genomics. It is also expected to reveal novel candidate salivary antigens for anti-tick vaccine development and thus to contribute towards a concerted effort for the control of a vector borne disease in Europe.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics
- natural sciences biological sciences biochemistry biomolecules proteins
- medical and health sciences basic medicine pharmacology and pharmacy pharmaceutical drugs vaccines
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2010-RG
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
370 05 Ceske Budejovice
Czechia
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.