Objective
Atherosclerosis is a chronic inflammatory response, clinically asymptomatic in the first decade. The deposition of lipids in arterial walls, due to the uptake of oxidized low density lipoproteins (oxLDL) by macrophages, causes blood flow restriction making cardiovascular diseases (CVD) a serious public health problem in EU countries. In oxLDL, oxidation of (phospho)lipids and proteins occurs, originating phospholipids-protein adducts by cross-linking reactions. Though phospholipid-protein adducts were identified in atherosclerotic plaques by immunoassays, their structural features were not yet described. It is known that smaller and denser (SD) oxLDL is more atherogenic, but the mechanism is yet unclear. Improved understanding of the pathology and potential therapies depends on molecular characterization of atherogenic LDL particles including the phospholipid-Apo B (PL-Apo B) adducts. To achieve this, mass spectrometry based strategies will be used to characterize PL-Apo B adducts in SD oxLDL from healthy and diseased clinical samples. The methodological approach will be developed in control LDL in vitro oxidized (HOCl, MPO, Me+H2O2) to determine the nature of PL-Apo B adducts formed, followed by biotinylation and separation by avidin magnetic beads of PL-Apo B adducts for optimization of PL-Apo B adducts isolation protocols with high specificity and sensitivity. Detection through MS strategies (MRM, CID) avoids time consuming analysis by a focused detection approach. Subsequently, the optimized approach will be applied to LDL from clinical samples in search of PL-Apo B adducts. The data from clinical samples will be scrutinized by chemometric techniques for discriminant ions prior to full characterization. This study will contribute to the molecular understanding of CVD pathology. The development of advanced methods for routine analysis will enable identification of new biomarkers for early clinical diagnosis.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- medical and health sciences health sciences public health
- medical and health sciences clinical medicine cardiology cardiovascular diseases arteriosclerosis
- natural sciences biological sciences biochemistry biomolecules lipids
- medical and health sciences basic medicine pathology
- natural sciences chemical sciences analytical chemistry mass spectrometry
You need to log in or register to use this function
We are sorry... an unexpected error occurred during execution.
You need to be authenticated. Your session might have expired.
Thank you for your feedback. You will soon receive an email to confirm the submission. If you have selected to be notified about the reporting status, you will also be contacted when the reporting status will change.
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2009-IEF
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
B4 7ET Birmingham
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.