Objective
The objective of my laboratory is to understand the mechanisms of mRNA polyadenylation and deadenylation, thereby providing fundamental insight into the regulation of eukaryotic gene expression. These objectives will be met through an integrated, multidisciplinary approach combining structural, biochemical, biophysical and yeast genetic techniques. Specifically, I propose to establish how mRNA poly(A) tail position and length are determined by the Cleavage and Polyadenylation Factor (CPF) and by the Ccr4-Not complex.
Gene expression is tightly regulated, both spatially and temporally. Traditionally, this has been studied through transcription but more recent work has shown that post-transcriptional mechanisms play extensive roles. Many of these post-transcriptional functions are mediated by poly(A) tails which are found on almost all eukaryotic mRNAs. The CPF and Ccr4-Not complexes influence poly(A) tails to regulate mRNA stability and the efficiency of translation. They also mediate the periodic expression of cell cycle-related genes, microRNA-targeted gene silencing and expression of maternal masked mRNAs in oocyte development. My work will address many of these functions and will have broad-ranging biological and technological implications including: a better understanding of gene expression and the newly-emerging field of post-transcriptional regulation, the development of new technologies to study multi-protein complexes and the identification of potential therapeutic targets.
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
ERC-2010-StG_20091118
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Host institution
W1B 1AL LONDON
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.