Objective
Our goal is to understand the innate immune response to HIV-1. We have found that a cryptic innate immune response to HIV-1 exists in dendritic cells (DC). This response is normally inactive because HIV-1 is unable to infect DC. We have used the Vpx protein found in other lentiviruses to render DC susceptible to HIV-1 infection. The resulting innate response to HIV-1 requires an interaction between de novo synthesized Capsid and cellular Cyclophilin A. This interaction leads to the IRF-3-dependant production of type-I interferon and the activation of antigen-specific T cells. This study will aim at understanding the molecular mechanisms controlling this response.
In Aim 1, we will determine the host and viral factors implicated in the innate response to HIV-1. At the host level, we will evaluate a panel of candidate genes using a novel RNAi strategy using shRNA vectors in DC. At the viral level, we will determine the minimal viral elements required for triggering the response.
In Aim 2, we will identify novel regulators of the innate immune response in human DC, including the DC-specific HIV-1 innate sensor. Using a DC cDNA library, we will screen for inducers of type-I interferon in cell lines. This library will also be used to identify the Vpx-sensitive HIV-restriction factor in human DC.
The cryptic nature of the innate response to HIV-1 may explain in part the failure of the immune system to control HIV-1 in the vast majority of infected individuals, as well as the failure of current vaccine strategies. This study will provide a novel understanding of the molecular mechanisms responsible for innate immune responses, and may provide new immunological strategies for controlling HIV-1 infection.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- natural sciences biological sciences biochemistry biomolecules proteins
- medical and health sciences basic medicine immunology
- medical and health sciences basic medicine pharmacology and pharmacy pharmaceutical drugs vaccines
- engineering and technology electrical engineering, electronic engineering, information engineering electronic engineering sensors
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2010-RG
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
75231 Paris
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.