Objetivo For decades, researchers have used small mammals to study the underlying processes of human disease and potential therapies for such ailments. Current strategies for measuring disease progression and/or drug effect usually involve groups of animals being killed at multiple time points for post-mortem tissue analysis. Recently, transgenic strains of mice have been created in which light-emitting luciferase is produced when a disease-specific pathway is switched on or off. High-sensitivity cameras are used to follow the profile of light emission in individual animals during disease induction/treatment, thus avoiding serial culling. However generation of transgenic mice is time-consuming, expensive, and costly in terms of animal usage. A major problem is that every tissue in the mouse may emit light, soorgan-specific effects are difficult to discern. This research programme describes the development of a process of generating light-emitting somatic-transgenic rodents by gene transfer in neonates or in utero. This permits the production of micecontaining bespoke biosensors and avoids the lengthy process of production of germline transgenics. Crucially, specific organs and tissues can be targeted by the appropriate choice of application route and gene transfer vector. This technique is highly adaptable to new developments in biology (for example, new signalling targets, new genetic regulators such as miRNA and improved light-emitting enzymes) and is applicable to any strain of mouse or rat. As proof-of concept several widely-used models of disease will be examined over the duration of this programme of work: i) LPS-induced lung inflammation ii) Bleomycin-induced lung fibrosis iii) LPS-induced liver inflammation iv) Carbon tetrachloride/ethanol-induced liver fibrosis v) collagen-induced arthritis vi) Cutaneous wound-healing following dorsal punch biopsy vii) Neonatal cerebral hypoxia/ischaemia Ámbito científico engineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringsensorsbiosensorsmedical and health sciencesclinical medicinerheumatologymedical and health sciencesmedical biotechnologygenetic engineeringgene therapyengineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringsensorsoptical sensorsnatural sciencesbiological scienceszoologymammalogy Programa(s) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Tema(s) ERC-SG-LS7 - Applied life sciences, biotechnology and bioengineering: agricultural, animal, fishery, forestry/food sciences; biotechnology, chemical biology, genetic engineering, synthetic biology, industrial biosciences; environmental biotechnology. Convocatoria de propuestas ERC-2010-StG_20091118 Consulte otros proyectos de esta convocatoria Régimen de financiación ERC-SG - ERC Starting Grant Institución de acogida UNIVERSITY COLLEGE LONDON Aportación de la UE € 1 212 503,97 Dirección GOWER STREET WC1E 6BT LONDON Ver en el mapa Tipo de actividad Higher or Secondary Education Establishments Contacto administrativo Greta Borg-Carbott (Ms.) Investigador principal Simon Nicholas Waddington (Dr.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos Beneficiarios (3) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo UNIVERSITY COLLEGE LONDON Aportación de la UE € 1 212 503,97 Dirección GOWER STREET WC1E 6BT LONDON Ver en el mapa Tipo de actividad Higher or Secondary Education Establishments Contacto administrativo Greta Borg-Carbott (Ms.) Investigador principal Simon Nicholas Waddington (Dr.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos QUEEN MARY UNIVERSITY OF LONDON La participación finalizó Reino Unido Aportación de la UE € 104 855,77 Dirección 327 MILE END ROAD E1 4NS London Ver en el mapa Región London Inner London — East Tower Hamlets Tipo de actividad Higher or Secondary Education Establishments Contacto administrativo Gerard Collins (Mr.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos ST GEORGE'S HOSPITAL MEDICAL SCHOOL Reino Unido Aportación de la UE € 153 912,26 Dirección CRANMER TERRACE SW17 0RE London Ver en el mapa Región London Inner London — West Wandsworth Tipo de actividad Higher or Secondary Education Establishments Contacto administrativo Steff Hazlehurst (Ms.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos