Cel For decades, researchers have used small mammals to study the underlying processes of human disease and potential therapies for such ailments. Current strategies for measuring disease progression and/or drug effect usually involve groups of animals being killed at multiple time points for post-mortem tissue analysis. Recently, transgenic strains of mice have been created in which light-emitting luciferase is produced when a disease-specific pathway is switched on or off. High-sensitivity cameras are used to follow the profile of light emission in individual animals during disease induction/treatment, thus avoiding serial culling. However generation of transgenic mice is time-consuming, expensive, and costly in terms of animal usage. A major problem is that every tissue in the mouse may emit light, soorgan-specific effects are difficult to discern. This research programme describes the development of a process of generating light-emitting somatic-transgenic rodents by gene transfer in neonates or in utero. This permits the production of micecontaining bespoke biosensors and avoids the lengthy process of production of germline transgenics. Crucially, specific organs and tissues can be targeted by the appropriate choice of application route and gene transfer vector. This technique is highly adaptable to new developments in biology (for example, new signalling targets, new genetic regulators such as miRNA and improved light-emitting enzymes) and is applicable to any strain of mouse or rat. As proof-of concept several widely-used models of disease will be examined over the duration of this programme of work: i) LPS-induced lung inflammation ii) Bleomycin-induced lung fibrosis iii) LPS-induced liver inflammation iv) Carbon tetrachloride/ethanol-induced liver fibrosis v) collagen-induced arthritis vi) Cutaneous wound-healing following dorsal punch biopsy vii) Neonatal cerebral hypoxia/ischaemia Dziedzina nauki engineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringsensorsbiosensorsmedical and health sciencesclinical medicinerheumatologymedical and health sciencesmedical biotechnologygenetic engineeringgene therapyengineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringsensorsoptical sensorsnatural sciencesbiological scienceszoologymammalogy Program(-y) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Temat(-y) ERC-SG-LS7 - Applied life sciences, biotechnology and bioengineering: agricultural, animal, fishery, forestry/food sciences; biotechnology, chemical biology, genetic engineering, synthetic biology, industrial biosciences; environmental biotechnology. Zaproszenie do składania wniosków ERC-2010-StG_20091118 Zobacz inne projekty w ramach tego zaproszenia System finansowania ERC-SG - ERC Starting Grant Instytucja przyjmująca UNIVERSITY COLLEGE LONDON Wkład UE € 1 212 503,97 Adres GOWER STREET WC1E 6BT LONDON Zobacz na mapie Rodzaj działalności Higher or Secondary Education Establishments Kontakt administracyjny Greta Borg-Carbott (Ms.) Kierownik naukowy Simon Nicholas Waddington (Dr.) Linki Kontakt z organizacją Opens in new window Strona internetowa Opens in new window Koszt całkowity Brak danych Beneficjenci (3) Sortuj alfabetycznie Sortuj według wkładu UE Rozwiń wszystko Zwiń wszystko UNIVERSITY COLLEGE LONDON Wkład UE € 1 212 503,97 Adres GOWER STREET WC1E 6BT LONDON Zobacz na mapie Rodzaj działalności Higher or Secondary Education Establishments Kontakt administracyjny Greta Borg-Carbott (Ms.) Kierownik naukowy Simon Nicholas Waddington (Dr.) Linki Kontakt z organizacją Opens in new window Strona internetowa Opens in new window Koszt całkowity Brak danych QUEEN MARY UNIVERSITY OF LONDON Zakończenie uczestnictwa Zjednoczone Królestwo Wkład UE € 104 855,77 Adres 327 MILE END ROAD E1 4NS London Zobacz na mapie Region London Inner London — East Tower Hamlets Rodzaj działalności Higher or Secondary Education Establishments Kontakt administracyjny Gerard Collins (Mr.) Linki Kontakt z organizacją Opens in new window Strona internetowa Opens in new window Koszt całkowity Brak danych ST GEORGE'S HOSPITAL MEDICAL SCHOOL Zjednoczone Królestwo Wkład UE € 153 912,26 Adres CRANMER TERRACE SW17 0RE London Zobacz na mapie Region London Inner London — West Wandsworth Rodzaj działalności Higher or Secondary Education Establishments Kontakt administracyjny Steff Hazlehurst (Ms.) Linki Kontakt z organizacją Opens in new window Strona internetowa Opens in new window Koszt całkowity Brak danych
