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Content archived on 2024-05-28

Role of Microtubule Regulators in Centriole Elongation

Objective

Centrioles are cellular organelles made of microtubules that are essential for centrosome, cilia and flagella assembly and as such are involved in a variety of cellular and developmental processes, including cell motility, division and polarity. Centriole biogenesis is first seen during S phase by the appearance of procentrioles that elongate as the cell cycle progresses to reach nearly full length in mitosis. Most mature centrioles in an organism have similar length, yet very little is known on how centriole length control is exerted. The purpose of the current project is to investigate centriole biogenesis, in particular centriole elongation, its kinetics and regulation. We will search for novel molecular players in this process via two complementary approaches. We will perform an RNAi screening of microtubule regulators in mammalian cells and check for their role in centriole elongation. In addition, we will perform pull-down assays using already known molecular players in centriole length control and search for new binding partners by mass spectrometry. These combined approaches will identify novel players in centriole biogenesis that we will further study using in vitro and in vivo (Drosophila) approaches, to better understand their mechanism of action and their relevance to the organism.

Fields of science (EuroSciVoc)

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Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

FP7-PEOPLE-2009-IEF
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Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

MC-IEF - Intra-European Fellowships (IEF)

Coordinator

FUNDACAO CALOUSTE GULBENKIAN
EU contribution
€ 148 783,60
Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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