Objective
Legionella outbreaks have great sanitary, economic and social implications. Diagnostic method use to prevent and control Legionella is bacteria culture. Available detection kits are based on DNA technology, unable to discriminate between viable and non-viable bacteria. On the contrary, RNA is widely used as a viable bacteria indicator. Although molecular based techniques, as PCR and qPCR, have become essential tools in genetic analyses due to its high sensitivity and accuracy, they show drawbacks as time-consuming and expensive. Recent development of integrated microfluidic systems has allowed time, volume and analysis cost reduction. An important application of microfluididc systems is the ability to efficiently process raw samples and perform the required analytical assays “on-chip”. There is no portable system to detect viable Legionella on-site in the market. This device will avoid sample pre-treatment therefore reducing data acquisition. Moreover, rapid result gathering will allow implementing a rapid plan of action for bacteria eradication. The project aims to develop a portable microfluidic device composed of a filtration and bacteria collection system and a disposable card (containing a microfluidic chamber for nucleic acids concentration, a microfluidic chamber for RNA amplification through NASBA, and a detection system based on a NALFIA strip). An essential part of PINVIALEG is the development of a portable device where the filtration system is integrated to a microfluidic based cartridge. Thus, the whole process of Legionella detection (from sample collection to results) can be performed on site. NASBA is an isothermal process, facilitating portable equipment production. Development of a NALFIA strip able to detect NASBA products as a reading system will make the device very simple to use by non-qualified personnel. A new innovative tool will be produced to detect viable Legionella on-site, with rapid result acquisition and easy to use.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules nucleic acids
- natural sciences biological sciences microbiology bacteriology
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences genetics RNA
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-SME-2010-1
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
48170 Zamudio
Spain
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.