Objectif This project aims to develop novel biocatalysts for the production of glycosides (NOVOSIDES). Glycosylated compounds have a wide range of applications, but very few enzymes are able to glycosylate small organic molecules cost-efficiently at the industrial scale. Therefore, glycosylation reactions catalysed by transglycosidases, glycoside phosphorylases and glycoside hydrolases will be explored in more detail. These enzymes catalyze the transfer a glycosyl group from a cheap and readily available donor substrate to a variety of acceptors. To exploit their full potential, the enzymes’ specificity and stability against high temperatures and the presence of organic co-solvents will be optimised by means of directed evolution.A large and diverse collection of enzymes will first be established by screening in natural environments and by the mining of public (meta)genome databases. The enzyme collection will then be screened for activity on a variety of representative acceptors from different chemical classes. This will allow the identification of the most promising enzymes for optimisation through semi-rational and random mutagenesis. The high-throughput screening of natural and variant enzymes will be performed with newly developed fluorescent probes, that allow fast and accurate measurements of carbohydrate-active enzymes in a direct and non-destructive assay.To achieve these ambitious goals, a complementary consortium of academia and industry has been formed that covers the whole range of required expertises. The economical potential of our technology will be demonstrated by the development and scale-up of selected glycosylation reactions at pilot-plant facilities. The produced glycosides will be actively marketed to potential end-users to promote the valorisation of the project’s results and to initiate future collaborations on novel target compounds. Champ scientifique natural sciencescomputer and information sciencesdatabasesnatural scienceschemical sciencescatalysisbiocatalysisnatural sciencesbiological sciencesgeneticsgenomesnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes Programme(s) FP7-KBBE - Specific Programme "Cooperation": Food, Agriculture and Biotechnology Thème(s) KBBE.2010.3.3-01 - Robust and novel biocatalysts for industrial applications - Call: FP7-KBBE-2010-4 Appel à propositions FP7-KBBE-2010-4 Voir d’autres projets de cet appel Régime de financement CP-FP - Small or medium-scale focused research project Coordinateur UNIVERSITEIT GENT Contribution de l’UE € 787 550,00 Adresse SINT PIETERSNIEUWSTRAAT 25 9000 Gent Belgique Voir sur la carte Région Vlaams Gewest Prov. Oost-Vlaanderen Arr. Gent Type d’activité Higher or Secondary Education Establishments Contact administratif Wim Soetaert (Prof.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée Participants (5) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire MIKROBIOLOGICKY USTAV AV CR V.V.I Tchéquie Contribution de l’UE € 472 600,00 Adresse VIDENSKA 1083 142 00 Praha 4 Voir sur la carte Région Česko Praha Hlavní město Praha Type d’activité Research Organisations Contact administratif Vladimir Kren (Mr.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée RIJKSUNIVERSITEIT GRONINGEN Pays-Bas Contribution de l’UE € 521 000,00 Adresse Broerstraat 5 9712CP Groningen Voir sur la carte Région Noord-Nederland Groningen Overig Groningen Type d’activité Higher or Secondary Education Establishments Contact administratif Jan Poutsma (Mr.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée FRIEDRICH-SCHILLER-UNIVERSITÄT JENA Allemagne Contribution de l’UE € 378 880,00 Adresse FÜRSTENGRABEN 1 07743 JENA Voir sur la carte Région Thüringen Thüringen Jena, Kreisfreie Stadt Type d’activité Higher or Secondary Education Establishments Contact administratif Alexander Schiller (Prof.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée BIO BASE EUROPE PILOT PLANT VZW Belgique Contribution de l’UE € 372 000,00 Adresse RODENHUIZEKAAI 1 9042 Desteldonk Gent Voir sur la carte Région Vlaams Gewest Prov. Oost-Vlaanderen Arr. Gent Type d’activité Other Contact administratif Sofia Vindevogel (Ms.) Liens Contacter l’organisation Opens in new window Coût total Aucune donnée Carbosynth Limited Royaume-Uni Contribution de l’UE € 152 800,00 Adresse Old Station Business Park 8 and 9 RG206NE Compton Voir sur la carte Région South East (England) Berkshire, Buckinghamshire and Oxfordshire Berkshire Type d’activité Private for-profit entities (excluding Higher or Secondary Education Establishments) Contact administratif Vanessa Eastwick-Field (Dr.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée
