Objectif Protein-protein interactions are keys to executing important cellular functions. We hypothesize that gain or loss of protein-protein interactions plays important roles in carcinogenesis. We propose a network-based approach to biomarker discovery that uses protein-protein interactions and regulatory transcriptional networks. We will use prostate cancer and gliomas as models. Prostate cancer is the most common form of cancer and the leading cause of cancer death among men in the developed countries. Glioblastoma multiforme (GBM) is the most common and most aggressive type of primary brain tumor and it GBM accounts for 52% of all primary brain tumor cases. Novel markers and therapeutic targets are still needed for these two cancers. We will be focusing on the androgen receptor protein-protein network in prostate cancer and TGF-beta mediated network in gliomas. Protein interactions will be extracted by text mining, from databases, and from structural data on protein complexes. To identify transcription factors and their targets in human, ChIP-Chip and ChIP-Seq experiments will be carried out. The resulting networks serve as a scaffold on which data of deregulated proteins derived from microarray and next-generation sequencing of patient cancer samples will be mapped. The perturbed subnetwork will be visualized with a new method that identifies and highlights network motifs and modules. Promising biomarker candidates will be further examined and validated experimentally. Where possible, candidates will be modelled using protein structural data on protein-protein complexes, providing the basis to design ligands that occupy binding sites in order to disrupt cancer-relevant interactions. Our proposal is highly innovative. We expect to identify key nodal points in the network, to which protein-protein interactions can be disrupted as a way to perturb or interfere with the network. These key nodal points will serve both as biomarkers and therapeutic targets. Champ scientifique medical and health sciencesclinical medicineoncologyprostate cancernatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsproteomicsnatural sciencescomputer and information sciencesdatabases Programme(s) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Thème(s) FP7-PEOPLE-2009-IRSES - Marie Curie Action "International Research Staff Exchange Scheme" Appel à propositions FP7-PEOPLE-2009-IRSES Voir d’autres projets de cet appel Régime de financement MC-IRSES - International research staff exchange scheme (IRSES) Coordinateur TECHNISCHE UNIVERSITAET DRESDEN Contribution de l’UE € 81 000,00 Adresse HELMHOLTZSTRASSE 10 01069 Dresden Allemagne Voir sur la carte Région Sachsen Dresden Dresden, Kreisfreie Stadt Type d’activité Higher or Secondary Education Establishments Contact administratif Sven Kreigenfeld (Mr.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée Participants (2) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire HITS GGMBH Allemagne Contribution de l’UE € 70 200,00 Adresse SCHLOSS WOLFSBRUNNENWEG 35 69118 Heidelberg Voir sur la carte Région Baden-Württemberg Karlsruhe Heidelberg, Stadtkreis Type d’activité Research Organisations Contact administratif Andreas Reuter (Prof.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée UNIVERSITETET I BERGEN Norvège Contribution de l’UE € 21 600,00 Adresse MUSEPLASSEN 1 5020 Bergen Voir sur la carte Région Norge Vestlandet Vestland Type d’activité Higher or Secondary Education Establishments Contact administratif Inger Gjesdahl (Ms.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée