Objetivo "The detection and quantification of protein biomarkers in biological samples lies central to proteomics, drug design, disease prognosis and therapeutic development. The generation of viable protein microarrays is, though, challenging. The first protein microarrays were built onantibodies. Unfortunately antibodies do not function well in the microarray format, because typically only a small fraction (20%) specifically recognizes the target protein. Current antibody based optical assays are commonly based on sandwich assays in which antigen binding to the immobilised antibody is detected through the use of a secondary, labeled, antibody. Though sensitive, this method is laborious and often requires a specifically-labelled secondary antibody for every antigen of interest.Labelling protocols are potentially perturbative, can also be time consuming and may lead to high background signals. Alternative protein receptive molecules are thus of considerable interest. In recent years, the host group has developed, with a team in Leeds, optical and electrical assays based on the use of peptide aptamers (highly specific protein receptors built into the surface of robust scaffold proteins). These can be immobilized with controlled surface orientation on a variety of surfaces. The aim of this proposal is to utilize this experience in developing highly sensitive electrical protein assays using capacitance and impedance (AC, DC, Faradaic and Non Faradaic). Through appropriate surface chemical methods such assays will be operable in complex fluid such as cell lysates and blood. Being electrical they are also readily multiplexed at comparatively low cost, enabling simultaneous detection of multiple targets. The linear range potentiallyaccessible within such arrays is considerable, as is the potential clinical benefit." Ámbito científico natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsproteomicsmedical and health sciencesbasic medicinemedicinal chemistry Programa(s) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Tema(s) FP7-PEOPLE-2010-IIF - Marie Curie Action: "International Incoming Fellowships" Convocatoria de propuestas FP7-PEOPLE-2010-IIF Consulte otros proyectos de esta convocatoria Régimen de financiación MC-IIF - International Incoming Fellowships (IIF) Coordinador THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD Aportación de la UE € 280 680,00 Dirección WELLINGTON SQUARE UNIVERSITY OFFICES OX1 2JD Oxford Reino Unido Ver en el mapa Región South East (England) Berkshire, Buckinghamshire and Oxfordshire Oxfordshire Tipo de actividad Higher or Secondary Education Establishments Contacto administrativo Stephen Conway (Dr.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos
