Coordinated regulation of transcription and pre-mRNA splicing at gene promoters

Project Information

pre-mRNA splicing

Grant agreement ID: 276425

Project closed

Start date 1 May 2011

End date 30 April 2013

Funded under

• Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)

Total cost € 162 742,40

EU contribution € 162 742,40

Coordinated by

MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV
Germany

Objective

"Accurate pre-mRNA splicing is a fundamental step for gene expression. SR proteins, a protein family of splicing regulators, play an essential role in the process by defining intron-exon boundaries and recruiting the spliceosome to carry out splicing. Pre-mRNA splicing occurs co-transcriptionally and it is now emerging that promoter identity modulates alternative splicing, possibly through the recruitment of SR proteins or through kinetic coupling by altering Pol II elongation rates. The aim of the present proposal is to investigate the relationship between promoter elements, differential SR protein recruitment to genes and splicing outcome.
The genome-wide recruitment of SR proteins to promoter elements will be investigated resorting to innovative approaches. SR proteins tagged with LAP-tag and expressed in ""BAC cell lines"" will be isolated with associated DNA sequences (ChIP) which will then be used to interrogate a genome-wide promoter microarray (ChIP-on-CHIP). This will reveal similarities and differences in recruitment to genes and gene regions among SR protein family. To test how promoter elements influence spliceosome assembly and splicing outcome, model-genes containing alternative and constitutively spliced exons will be created. The focus will be on genes relevant for neuronal differentiation. Model genes will be introduced in cells already expressing individual SR proteins BACs, creating ""Double-BAC cell lines"". The results expected from this project will provide a global view of SR protein role in regulating and coupling transcription and pre-mRNA splicing. Elucidating these regulatory mechanisms is key for the understanding of genomic and proteomic diversity."

Fields of science (EuroSciVoc)

CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.

• natural sciences › biological sciences › genetics › DNA
• natural sciences › biological sciences › biochemistry › biomolecules › proteins

You need to log in or register to use this function

Programme(s)

• FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)

Topic(s)

• FP7-PEOPLE-2010-IEF - Marie-Curie Action: "Intra-European fellowships for career development"

Call for proposal

FP7-PEOPLE-2010-IEF
See other projects for this call

Funding Scheme

MC-IEF - Intra-European Fellowships (IEF)

Coordinator

MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV

• EU contribution: € 162 742,40
• Address: HOFGARTENSTRASSE 8 ; 80539 Munchen ; Germany
• Region: Bayern › Oberbayern › München, Kreisfreie Stadt
• Activity type: Research Organisations
• Total cost: No data