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Content archived on 2024-06-18

Regulation of chromatin compaction in response to DNA damage in mammalian cells

Objective

"Protection of genome integrity is crucial for maintaining cell viability and preventing cancer. However, how the dynamic organisation of DNA into chromatin in the cell nucleus is orchestrated in order to allow and promote detection and repair of DNA lesions remains poorly understood. A controlled accessibility of damaged chromatin is likely critical for an efficient cellular response to DNA damage, but underlying molecular mechanisms enabling this regulation have to be deciphered. In this proposal, we describe how we will combine established techniques and a novel approach based on FLIM-FRET microscopy to quantitatively visualise chromatin compaction/decompaction in human cells and to analyse how this is regulated during the response to genotoxic stress. First, we will examine the spatio-temporal dynamics of chromatin re-organisation upon global or localised DNA damage. Then, we plan to characterise the factors involved in each step, with a particular focus on chromatin remodeling factors, in order to determine their exact functional importance for DNA damage signaling and repair. This study should provide mechanistic insight into how genome integrity is preserved in a chromatin context."

Fields of science (EuroSciVoc)

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Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

FP7-PEOPLE-2010-IEF
See other projects for this call

Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

MC-IEF - Intra-European Fellowships (IEF)

Coordinator

INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE
EU contribution
€ 185 248,00
Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

No data

Participants (1)

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