Objective
The nuclear pore complex (NPC) is one of the most intricate multi-protein assemblies found in eukaryotic cells encompassing ~30 different components called nucleoporins (Nups). The structural determination of the NPC represents a major challenge due to its size and location in the nuclear envelope. Accurate quantitative data on NPC composition that are required to generate structural models at atomic resolution are currently lacking. Here, I propose to apply targeted mass spectrometry to study the stoichiometry and intracellular distribution of the human NPC components. For this purpose, I will establish an assay based on selected reaction monitoring (SRM) experiments that will allow the absolute quantitation of Nups in different cell compartments. The assay will be used to systematically analyze the effect of Nup perturbation on NPC composition by screening cell lines where single Nups are depleted by gene silencing. The compositional analysis of the gene silencing phenotypes will provide the base for complementary structural investigations performed using cryo-electron tomography. The integration of proteomic and structural data will provide accurate information on the stoichiometry and spatial arrangement of Nups within NPCs. In addition, by mapping the intracellular distribution of Nups, it will be possible to gain insights into their functional roles beyond transport. The multidisciplinary approach that will be developed has the potential to pave the way towards the integration of quantitative proteomic technologies in structural studies of other cellular substructures. This fellowship will give me the opportunity to lead an ambitious project at the interface between proteomics and structural biology, and to work in a collaborative interdisciplinary environment. The scientific and complementary skills I will acquire and the opportunity to work in a leading European institution will be fundamental for my development towards an independent scientific career.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins proteomics
- natural sciences physical sciences optics microscopy electron microscopy
- natural sciences chemical sciences analytical chemistry mass spectrometry
- natural sciences biological sciences molecular biology structural biology
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2010-IEF
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
69117 Heidelberg
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.